in vitro antioxidant activity of plant extracts pdf
0.2mM solution of DPPH in methanol was prepared. Materials and Methods: Herbal extraction was carried out by Soxhlet method using chloroform, ethylacetate, methanol, ethanol, aqueous and hydroalcohol. This study was c onducted to investigat e the antioxidant effect of the differen t crud e extrac ts (Pe troleum e ther, e thanolic a nd. This study aimed to investigate in vitro antidiabetic and antioxidant activity of three plants from Ficus species and effect of extracting solvents, total flavonoids and phenolics content on its in vitro activity.
Total antioxi-dant activity of ethanol extracts of plants were also tested by using ferric thiocyanate (FTC) and thiobarbituric acid (TBA) methods. [32,33,34,35,36,37,38] Further examination by bio-autography is ongoing in order to determine new .
Proteinase activity was also significantly inhibited by the leaf (84.19 g/ml) and stem (81.84 g/ml).
Scavenging activity was tested by 1,1-diphenyl-2-picrylhydrazyl (DPPH) method and the inhibitory effect on lipid peroxidation was examined by the ferric thiocyanate (FTC) and thiobarbituric acid (TBA) methods.
The aim of this study was to screen various solvent extracts of whole plant of Torilis leptophylla to display potent antioxidant activity in vitro and in vivo, total phenolic and flavonoid contents in order to find possible sources for future novel antioxidants in food and pharmaceutical formulations.. Material and methods. Both aqueous extracts inhibited -glucosidase activity in vitro; this inhibition of the enzyme could slow down the breakdown of disaccharide to simple glucose, hence reducing the amount of glucose absorbed in the blood (Kwon et al., 2006).
The obtained results . The antioxidant activity of plant extracts was determined by different in vitro methods such as the DPPH free radical scavenging assay and reducing power methods.
4. Result from this assay clearly showed that all extract exhibited high antiradical activity towards DPPH radical. 2.1.
The antiproliferative and antioxidant potential of Curcuma aeruginosa extracts were investigated. download download pdf.
However, both extracts exhibited a dose-dependent enzyme inhibitory activity in the range of extract had reducing power but not at same level and the activity is ranging from 42.24 - 62.65 % Statistical correlations have been studied between respectively at a concentration of 1mg ml-1 in total phenolic content and antioxidant activity reaction mixture.
of antioxidants in treating diabetes and its complications through prevention of oxidative stress has been explained in different studies.20,24-26 Interestingly, the current study revealed that Bersama abyssinica leaves have strong in vitro antioxidant activities indicating that the plant could possess antidiabetic activity.
Plantago major plant showed the highest antioxidant capacity compared to other plant extracts in results of the in-vitro assays including 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging . Free radicals are the primary cause of oxidative damage of biological molecules in the human body, and are related to coronary heart disease, aging, cancer, and dementia .We used the 2,2-diphenyl-2-picrylhydrazyl (DPPH) assay to determine the antioxidant activity of cocoa plant methanolic extracts, i.e., their ability to scavenge free radicals; concentrations of . In Vitro Antioxidant Assays The antioxidant activity of methanolic leaf extract was determined by different invitro methods such as, DPPH free radical scavenging assay, nitric oxide assay and reducing power methods.
In this study, aqueous extracts of some medicinal and aromatic plants (garlic, Turkish Oregano, rosemary, basil, and peppermint) were used in probiotic yogurt production to increase functionality of probiotic yogurt.
The use of plant peels has dual benefits; first is their antimicrobial activity against food-borne pathogens, while the second is minimizing agro-industrial wastes. Recently, numerous studies reported the antioxidant properties of medicinal plant products using DPPH assay [42-44].
Reducing power activity of different plant important natural antioxidant [49,50]. All seven plants (100%) inhibited the growth of at least one tested foodborne and spoilage bacteria and fungi.
spores were also affected. These fractions were subjected to Total antioxidant assay.
The antioxidant activities (AA), reducing powers (RP) and total phenolic contents (TPC) of methanol and water extracts of three lichen species, Usnea longissima Ach., Usnea florida (L.) Weber ex Wigg.
The antioxidant activity is expressed as the number of equivalents of ascorbic acid and was calculated by the following equation: A = (c V) / m 20 Scholars Research Library Mohammad Shahriar et al Annals of Biological Research, 2015, 6 (4):19-26 _____ Where, A = total content of Antioxidant compounds, mg/gm plant extract, in Ascorbic acid . extract showed increased reducing power with increase in concentration.
In the Far North Region of Cameroon, pastoralists use the leaves of Boscia senegalensis and the stem of Tapinanthus dodoneifolius to treat common animal diseases.
Ferric Reducing Antioxidant Power (FRAP) Generally, the methanolic extracts of three studied medicinal plants exhibited remarkable concentration-dependent increases in absorbance values at a wavelength of 700 nm (Table 2).At all the tested concentrations, the extracts of A. hockii had significantly higher absorbance values than those recorded for C. volkensii .
Objective: The aim of the present study is to investigate the antioxidants and the antibacterial activities of the aerial part extracts of this plant. Here, 70% ethanol extracts of 22 HPs collected from along the coast of South Korea were investigated for their potentials of antioxidant, anti-aging, and whitening properties for use as materials in novel cosmeceuticals. The results of the free radical scavenging activity of Argel extract and the authentic antioxidant L-ascorbic acid was summarized in Table (1). Cordiamyxa fruit extract exhibits low cytotoxicity, antibacterial activity, and antioxidant properties in vitro and can be developed for pharmaceutical and medical uses in the future. The aim of our study was to evaluate in vitro antioxidant potential of methanolic extracts (ME) of 14 medicinal plants, 8 of which are endemic species of Anatolia.
Abstract: The present study investigated antioxidant activities of Leonotis leonurus extract both in vivo and in vitro to provide scientific basis to traditional usage of this plant.
Total polyphenols and total antioxidant activity of the Dahlia x hybrida flower extracts.
Of the extracts tested, the methanol e g. pramodini. Aqueous extracts of turmeric showed antioxidant and antimicrobial activity due to the presence of curcumin (5%), a polyphenolic compound.
Free radical scavenging activity The percentage (%) free radical 1,1-diphenyl-2-picrylhy- drazyl (DPPH) scavenging activity of extracts was assessed as previously described by Hatano et al  and was calculated as follows: % radical scavenging effect Abs1 Abs2 =Abs1 100 where Abs1 is the absorbance of the control, and Abs2 is the . The ethanol extracts exhibited more significant antioxidant activity than hexane/acetone extracts. The ABTS method is known to be a rapid method for the determination of the antioxidant activity and could be a useful tool to screen samples and cultivars in order to obtain high content of natural antioxidants in foods .The ABTS + scavenging activity is presented in the Table 4.This results show that at all the concentration, the AEE showed a higher inhibitory potential compared to other . The hexane/acetone extracts were by far the best antimicrobial agent . The reaction mixture consists of 1 ml of plant extract (1mg/ml), 1 ml of PMS (60m) preprared in The evaluation of the antimicrobial potential of both methanolic and ethanolic extracts of three fruit . The extract dissolved in methanol (0.5 mg/ml). Background.
Generally, plant- or fruit/vegetable-derived foodstuffs are rich in phenols and exhibit an excellent antioxidant capacity, partic- ularly phenols that can give the hydrogen atoms in their hydroxyl groups (27). Herein, the CMF ethanol extract was also evaluated for antioxidant activity.
The . one of the stable free radicals, commercially available, nitrogen centered, and largely used for evaluat-ing scavenging activity of antioxidant standards and plant extracts with a characteristic absorbance at nm , The results suggested that the strong antioxidant activity of extracts might be due to the presence of phenolics compounds present in the extract .
500 l of Table 2.
Our aim was to evaluate in vitro antioxidant activity of the crude methanolic extracts (ME) of 14 medicinal plants, 8 of which are endemic species to Anatolia, by commonly used methods [1,1-diphenyl-2-picrylhydrazyl (DPPH), ferric thiocyanate (FTC) and thiobarbituric acid (TBA) methods]. DPPH radical scavenging activity The free radical scavenging activity by different plant extracts was done according to the method reported by (Gyamfi et al, 2002)3.
In this study, the ethanol extracts of 18 Ethiopian wild medicinal plants were investigated for their in vitro antioxidant and anti-proliferative potential. Inhibition of Acetylcholinesterase (AChE) is still considered as the main therapeutic strategy against Alzheimer's disease (AD). Antioxidative activities of plant extracts were found
Materials and methods Plant selection and collection
The use of natural preservatives became of great interest; good examples of these natural preservation agents are plant peels.
The in vitro antioxidants activity was evaluated by determining ferric reducing power, total flavonoids, phenolics, flavonols and proanthocyanidins contents using . Herein, the CMF ethanol extract was also evaluated for antioxidant activity.
SCF extracts were subjected to column chromatography was resulted in six fractions. In this study, the antioxidant and anthelmintic activities of aqueous and ethanolic extracts of the leaves of Theobroma cacao were investigated.
The extracts were isolated by Super Critical fluid extraction method.
Aim: To evaluate the antioxidant potential and free scavenging activity of T. zeylanicum powdered root extract. From these assay several molecules from medicinal plants were . The different extracts were dissolved in ethanol at a concentration of 50-200 mg/ml.
Different concentrations of plant extract (100 2000 g/ l) in 1 ml of methanol were mixed with a phosphate buffer (2.5 ml, 0.2 M, pH 6.6) and The leaf and stem ethanol extract also showed in vitro anti-inflammatory activity by inhibiting the heat induced albumin denaturation and red blood cells membrane stabilization with 87.14 and 86.76 and 78.11, 74.17 g/ml respectively. However, the activity of the extract (0.84510.001) was lower than the standard (1.07940.010).
In Vitro analysis of Different extracts of Carica papaya (leaves) and their Phytochemical constituents, Antibacterial activity and Antioxidant activity June 2022 DOI: 10.15515/abr.0976-4585.12.4 . 6. The free radical scavenging activity of plant extract of Cressa cretica was studied on in vitro antioxidant models.
The in vitro antidiabetic, antioxidant activity, total phenolic compound content and phenolic compounds of yogurts were evaluated during the 28 day of storage period.
Phenolic plant extracts are sources of natural bioactive compounds, which can inhibit the rate of food spoilage.
Journal of Plant Science and Research 2013.
For this purpose DPPH (2,2-diphenyl-1-picrylhydrazyl), ORAC (oxygen radical absorbance capacity) and TPC (total phenolic content) assays together with MTT cell viability assay (performed on . In the present study, methanolic extracts of 20 plants used in Indian Ayurvedic system of medicine for improving cognitive function were . The final extract thus obtained was used for in vitro antioxidants activity.
However, both extracts exhibited a dose-dependent enzyme inhibitory activity in the range of Antioxidant Activity 3.2.1. Abstract: Introduction: Ficus religiosa, Ficus benghalensis and Ficus glomerata are plants from Ficus species used traditionally for the treatment of various ailments.
(2010) with slight modifications was used. Theobroma cacao L. (Sterculiaceae) leaves have been used in folklore medicine as treatment for wound healing, worm expellers and malaria cure. Abs sample is the absorbance of DPPH radical + sample (i.e.extract or standard) Scavenging activity of superoxide anion: The scavenging activity of the superoxide anion was determined by the method of Yen and Chen 17. Abstract: Objective: Screening of preliminary phytochemicals, evaluation of in vitro antioxidant and in vitro anticancer activities of Simarouba glauca leaf extracts on T-24 Bladder cancer cell line.
Extracts Orange colour . In vitro antioxidant activity. and Lobaria pulmonaria (L.) Hoffm. .
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Leaves | Context: Oxidative stress and . Halophyte plant (HPs), a salt-resistant flora, has been reported to provide several health benefits, but the knowledge of its cosmeceutical potential is still ambiguous.
Search results . Antioxidative activities of plant extracts were found
Different concentrations of plant extract (100 -2000 g/ l) in 1 ml of methanol were mixed with a phosphate buffer (2.5 ml, 0.2 M, pH 6.6) and potassium ferrocyanide (2.5 ml, 1 %).
It is known that the phenolic character of curcumin is responsible for its anti-oxidant properties . Medicinal plants, as source of remedies, are widely used as alternative therapeutic tools for the prevention or treatment of many diseases. Request PDF | In Vitro Antioxidant, Cytotoxic, Thrombolytic Activities and Phytochemical Evaluation of Methanol Extract of the Ampelocissus Barbata (Wall.) Jan Tauchen, Ivo Doskocil, Cecilia Caffi, Ermias Lulekal Molla . One of the fractions showed comparatively high antioxidant activity.
Antioxidant activity was based on the absorbance on the final day of FTC method.
Fresh root .
Recent investigation has shown that many flavonoids and related polyphenol contribute significantly to the antioxidant activity of many fruits such as red grape, vegetables and medicinal plants . is traditionally known to be used as an anti-infective, expectorant, healer of wounds and against inflammatory and gastrointestinal diseases. The phytochemicals (tannins, phenols, saponins, terpenoids, flavonoids and glycosides) present in the leaves . our plant extracts, the ability to scavenge the stable free . Regarding this ethnopharmacological study allowed to compare the antimicrobial activities of various plant species, to establish the antimicrobial spectra of standardized plant extracts, and give support to the traditional use of these plants.
1,2 Accumulation of free radicals can cause pathological conditions such as . The antioxidant activity was evaluated by determining the activity of hydrogen peroxide (H 2O2) radicals scavenging and 1,1-diphenyl-2-picryl hydrazyl (DPPH) assay.
In this study, the ethanolic extract of the plant C. maritima was used for the determination of TPC, TFC, antioxidant activity, and ex vivo anti-cataract activity using goat eye lenses to know its potential for the effective treatment of cataract.
The protective effects of plant extracts were compared with a well known antioxidant, Butilated Hydroxytoluen (BHT) and -tocopherol.
showing the antioxidant property of S. trifasciata ethanolic and aqueous leaf extracts. Many plant derived phytochemicals have shown AChE inhibitory activity in addition to the currently approved drugs for AD.
Material and Methods: The plant extract (50mg) obtained by soxhlet extraction method was dissolved separately in 50ml of methanol and the resultant solution serially diluted to concentrations 0.5, 0.25, 0.125 and
2.4 | In vitro antioxidant assays 2.4.1 | DPPH scavenging activity The method of Shen et al.
Phytochemical components, including the phenolic and flavonoids, are important compounds that determine the plants antioxidant capacity, mainly due to their redox properties. Although many of these species are nearly facing extinction .
and has been reported to possess antioxidant properties both in in vitro and animal studies. In vitro antioxidant and ex vivo anti-cataract activity of ethanolic extract of Cineraria maritima: a traditional plant from Nilgiri hills Sumit Durgapal1,2*, Vijay Juyal1 and Anurag Verma2,3 Abstract Background: Cineraria maritima has a long history of use in the treatment of cataract and other eye-related problems in the homeopathic system of . Ismail Hossain, Farzana Anwar Sharmin, Sadika Akhter, Mohiuddin Ahmed Bhuiyan, *Mohammad Shahriar Department of Pharmacy, University of Asia Pacific, Dhanmondi, Dhaka, Bangladesh INTRODUCTION For decades, the utilization of herbal plants has Estimation of total phenolic content Total pheno lic cont ent was est imated using the Folin-Ciocalteu method  .
Due to the climate diversity in Tunisia, the traditional pharmacopoeia consists of a wide arsenal of medicinal plant species since long used in folk medicine, in foods as spices, and in aromatherapy. To achieve this, four extracts (water, methanol, chloroform and hexane) of both plants obtained by successive .
This study aimed to evaluate the in vitro antibacterial, non-cytotoxic and antioxidant potentials of these plants.
comparative study on phytochemical screening and antioxidant activity on ethanolic extract of actinidia deliciosa and vitis vinfera qr code. 3(1): 13-15.
Generally, plant- or fruit/vegetable-derived foodstuffs are rich in phenols and exhibit an excellent antioxidant capacity, partic- ularly phenols that can give the hydrogen atoms in their hydroxyl groups (27).
21,5 The high antioxidant activity of Ephedra alte extract can be explained by the presence of the hydroxyl groups in the phenolic compounds.
Download "(. In all these studies, a significant correlation existed between The antioxidant activity of . Both aqueous extracts inhibited -glucosidase activity in vitro; this inhibition of the enzyme could slow down the breakdown of disaccharide to simple glucose, hence reducing the amount of glucose absorbed in the blood (Kwon et al., 2006).
The recent studies have investigated that the antioxidant effect of plant products is mainly attributed to phenolic compounds such as flavonoids, phenolic acids, tannins etc. The HRBC membrane stabilization method is mainly used to estimate the anti-inflammatory activity of plant extracts 110.
Anti-diabetic property of the plant was observed with a significant result of inhibiting a maximum of 83% of -amylase activity (700g/ml concentration of methanol extract) whereas antioxidant .